Bailey butel
Clostridium sporogenes (anaerobes)- a 48 hours thioglycollate broth culture.
Robertson’s cooked meat medium/ Thioglycollate broth.Requirements for Culture of anaerobic bacteria An indicator also adds to demonstrate anaerobiosis. The resulting reaction liberates hydrogen and carbon dioxide. Water is added to the sachet and it is immediately placed in the jar, which is then sealed tightly.
The catalyst, consisting of pellets of sodium borohydride, cobalt chloride, citric acid, and sodium bicarbonate is contained in the sachet. This utilizes a transparent polycarbonate jar with a lid bearing a screened catalyst chamber. They include the McIntosh and Fildes anaerobic jar, which has inlets to admit hydrogen and carbon dioxide, a vacuum pump for evacuating oxygen, and a catalyst fitted into the lid. Similarly, a simpler but more expensive technique is the Gaspak system. Anaerobic jars are a constant feature of anaerobic culture. A burning candle can not continue due to the exhaustion of oxygen inside the jar. In laboratories, combustion involves the combining of oxygen with hydrogen to form water in the presence of a catalyst like palladium or palladinized asbestos. Robertson’s cooked meat medium. Another simple method of anaerobiosis in the application of a candle jar. Cooked meat particle also acts as s good reducing agent due to having glutathione e.g. Strict anaerobes will not grow within a centimeter of the surface. Using reducing agents like glucose, ascorbic acid, cysteine, thioglycollate in the medium.
Anaerobes grow in the depth of the medium, and the number of colonies becomes fewer towards the surface.